BACKGROUND: Vitreous chamber volume (VCV) in humans can be accurately measured using magnetic resonance imaging (MRI) combined with ITK-SNAP analysis software. This study explores the relationship between VCV and axial length (AL). METHODS: This study included 46 healthy individuals, encompassing a total of 92 eyes. Individuals with a history of eye conditions were excluded from the study. AL measurements were obtained using the Lenstar 900 (Haag-Streit, Switzerland). VCV was assessed through MRI using the Ingenia 3.0T system (Philips Medical Systems, Netherlands), with VCV analysis performed using ITK-SNAP analysis software version 3.8.0. The correlation between AL and VCV was evaluated using Pearson linear correlation coefficient, and a logistic regression model was employed to predict VCV. RESULTS: The sample consisted of 92 eyes from 46 individuals, including 28 women and 18 men. The average VCV was 6193±1135.9 mm3 for women and 6324.3±886.7 mm3 for men. The mean AL for women was 24.70±2.78 mm, while for men it was 24.66±1.70 mm. A significant correlation between AL and VCV was observed (P<
0.001). CONCLUSIONS: VCV can be accurately estimated through AL measurements, offering a clinically relevant method for assessing VCV.