This study aimed to evaluate the efficiency and accuracy of autotransplantation of complex impacted teeth by CAD/CAM digital navigation technology. An 11-year-old girl with complex impacted second premolars bilaterally in the maxilla was selected. CAD/CAM digital dynamic navigation was utilized to ensure the precision and success of the procedure. A comprehensive accuracy analysis was conducted to compare the preoperative digital simulation design with the postoperative positions of the transplanted teeth. The transplanted teeth functioned well after 1 year of follow-up. Postoperative CBCT clinical data, combined with Mimics 21.0 and 3-Matic 13.0 software, revealed that the depth and buccolingual deviations of the bilateral autotransplantation were within 2.0 millimeters, with a mesiodistal deviation of approximately 1.0 millimeter. This case demonstrates the high efficiency, precision, and stabilization provided by digital navigation technology, highlighting its potential as a viable option for the treatment of complex impacted teeth.