A paradigm shift: analytical ultracentrifugation as a multi-attribute platform method in targeted protein degradation.

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Tác giả: Gekleng Chhor, Michael T DeLion, Aaron C Ehlinger, Michelle A Estrada, Suki M Hyman, Lake N Paul, Justin M Reitsma, Judith A Ronau, Vladimir Stojkovic, Tiffany A Thibaudeau, Anil Vasudevan, Scott E Warder, Alexander E Yarawsky

Ngôn ngữ: eng

Ký hiệu phân loại: 331.25725 Conditions of employment

Thông tin xuất bản: Germany : European biophysics journal : EBJ , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 183327

Targeted protein degradation (TPD) has garnered appreciable interest in drug discovery due to its unique mechanism of action - degradation of a target in an event-driven manner, instead of traditional occupancy-driven inhibitor-based therapies. This is achieved by employing mono- or hetero-bifunctional small molecules known as degraders to induce the proximity of two proteins: a target protein and an E3 ubiquitin ligase, ultimately resulting in clearance of the target protein by the cell's inherent degradation machinery. A critical step in this pathway is ternary complex formation (TCF) between the ligase, degrader molecule, and the target protein. Although a bevy of biochemical, biophysical, cellular and structural approaches have been used to characterize degrader-induced ternary complexes, several knowledge gaps remain, such as stoichiometry and how much ternary complex is formed in solution. Analytical ultracentrifugation (AUC) is a biophysical method that is uniquely suited to address these questions, yet to this point has been surprisingly overlooked as an ideal method to characterize degrader candidates. In this study, we leveraged sedimentation velocity AUC (SV-AUC) to profile the degrader-induced ternary complex formation between Bruton's tyrosine kinase (BTK) and Cereblon (CRBN), allowing for evaluation of multiple attributes including sample purity, percent ternary complex, binding and kinetic rate constants, and hydrodynamics. We show that sedimentation equilibrium AUC (SE-AUC) can further complement the SV-AUC data with accurate molecular weight estimates of the ternary complex to confirm stoichiometry. This work demonstrates that AUC can be used both as a highly informative platform method for rapid characterization of candidate degrader compounds and as a rigorous method for elucidating additional details of the system.
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