OBJECTIVES: This is the first study conducted in southern Spain to determine i) the population structure (PS) of carbapenem-resistant (CR) Acinetobacter baumannii isolates by multilocus sequencing typing (MLST) and core genome MLST (cgMLST) and ii) the association between the sequence type ST and the bla METHODS: Of 336 isolates submitted to the Andalusian reference laboratory (PIRASOA
December 2017-2020), 73 were subjected to WGS (MiSeq). The following analyses were performed: bacterial identification (ribosomal MLST), carbapenemase gene detection (Resfinder 4.0), PS delineation (MLST by MLSTfinder 2.0 and cgMLST by Ridom SeqSphere RESULTS: The carbapenemases detected were bla CONCLUSIONS: The PS of CR A. baumannii in Andalusia is characterized by a dominant ST2/bla