BACKGROUND: Micronutrients (folate and vitamin B METHODS: A total of 200 pregnant women (100 Non-GDM and 100 GDM) were included in the study and blood samples were collected at 4 different time intervals throughout pregnancy (Visit-1 = 11-14 weeks, Visit-2 = 18-22 weeks, Visit-3 = 26-28 weeks, and Visit-4 = at delivery). RESULTS: Vitamin B CONCLUSIONS: Elevated micronutrients and lower homocysteine levels in GDM women necessitate more research for better understanding the role of these one carbon nutrients in the etiology of GDM and emphasizes the need to establish the optimum maternal vitamin B