[Contamination of Conjugative Antibiotic-resistant Plasmids in Large-scale Livestock and Poultry Manure and Their Occurrence Characteristics of Antibiotic Resistance Gene].

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Tác giả: Hao-Ze Gao, Ya-Jie Guo, Ying Li, Tian-Lei Qiu, Lei Shen, Xu-Ming Wang, Guo-Zhu Zhao

Ngôn ngữ: eng

Ký hiệu phân loại: 271.6 *Passionists and Redemptorists

Thông tin xuất bản: China : Huan jing ke xue= Huanjing kexue , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 184739

The emergence and spread of antibiotic resistance in bacterial pathogens are now becoming a serious threat to public health globally. To study the occurrence and transfer characteristics of plasmid-mediated mobile antibiotic resistance genes (ARGs) in manure, antibiotic-resistant plasmids were captured from layers, broilers, and pig farms in Beijing, Hebei, and Ningxia provinces. The conjugative antibiotic-resistant plasmids (CARP) were captured by a filter membrane conjugation experiment, and the transfer frequency of plasmids from feces to recipient bacteria was tested. The ARGs-carried plasmid was extracted and sequenced with the Illumina sequencer, and the replicon types of CARP were identified with the PlasimidFinder database. The antibiotic-resistant phenotype of the captured conjugative plasmid was determined using the disk diffusion method (Kirby-Bauer). In general, the 125 CARPs that were captured from 35 farms carried 13 types and 65 subtypes of ARGs. The most common ARGs were
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