BACKGROUND: Vascularization of the distal latissimus dorsi (LD) muscle flap determines the success of the procedure, particularly in large defects. Failure due to distal flap necrosis can necessitate reoperation and increase mortality. Indocyanine green fluorescence angiography (ICGFA), as a modality that allows for intraoperative imaging of fasciocutaneous flap perfusion, has revolutionized free flap surgery. Evidence of use in muscle flap perfusion assessment is lacking. We investigate the efficacy of ICGFA in predicting distal flap necrosis in large LD free flaps. METHODS: We prospectively recorded all cases of large LD free flap reconstruction (surface area >
250 cm RESULTS: A total of 107 patients with mean age of 57 ± 18 years and BMI of 29 ± 6 kg/m CONCLUSION: ICGFA is associated with increased success of large LD free flap reconstruction, allowing a more accurate and reliable assessment of perfusion. This highlights the immense potential of ICGFA as a clinical standard, surpassing its application solely in fasciocutaneous free flap surgery and, showcasing its efficacy in free muscle flap procedures. REGISTRATION: This study has been registered at https://www.researchregistry.com (identification number: researchregistry9496).