Hepatitis A virus (HAV) is the predominant cause of acute viral hepatitis worldwide
however, data on HAV prevalence among schoolchildren are limited. This study aimed to investigate HAV seroprevalence among schoolchildren in Amran governorate. This is a cross-sectional study that was carried out among 299 students between October 2023 and July 2024. The blood specimens were collected, and the anti-HAV antibody was detected by an enzyme-linked immunosorbent assay (ELISA) technique. Of 299 students screened, the seropositivity rate for HAV was 4 (1.3%). A higher rate of HAV was detected among male students 3 (1.6%), in the age group of 5-8 years 1 (11.1%), and in those whose parents had a secondary certificate 2 (2.3%). Moreover, a higher seroprevalence of HAV was observed among students who drank untreated water (1.6%) and those with a history of hepatitis A infection (6.7%). Additionally, this result revealed that children whose families had a history of HAV infection had the highest risk factors for HAV infection (OR = 8.9667), and those whose family members had an HAV infection (OR = 1.9029). To combat the HAV requires the implementation of preventive measures aimed at improving hygiene and sanitation, advocating widespread vaccination, and a rapid response to outbreaks of the virus.