Sonication-assisted protein extraction improves proteomic detection of membrane-bound and DNA-binding proteins from tumor tissues.

• Tác giả: Lijun Chen, David J Clark, Qing Kay Li, T Mamie Lih, Michael Schnaubelt, Hui Zhang
• Ngôn ngữ: eng
• Ký hiệu phân loại: 573.58 *Hair and fur
• Thông tin xuất bản: England : Nature protocols , 2025
• Bộ sưu tập: NCBI
• ID: 185245

Tóm tắt

Deep-scale, mass spectrometry-based proteomic studies by the Clinical Proteomic Tumor Analysis Consortium (CPTAC) program involves tissue lysis using urea buffer before data acquisition via mass spectrometry for quantitative global proteomic and phosphoproteomic analysis. This is described in a 2018 protocol