To enhance the sensitivity, integration, and practicality of the Raman detection system, a deep learning-based dual-functional subregional microfluidic integrated hydrogel surface-enhanced Raman scattering (SERS) platform is proposed in this paper. First, silver nanoparticles (Ag NPs) with a homogeneous morphology were synthesized using a one-step reduction method. Second, these Ag NPs were embedded in