OBJECTIVES: Accurately quantifying low-density lipoprotein cholesterol (LDL-C) is crucial for precise cardiovascular disease risk assessment and treatment decisions. The commonly used Friedewald equation (LDL-C METHODS: Using data from three studies comprising 5,738 datasets, LDL-C was determined with the four methods in samples with TG up to 5.65 mmol/L. We calculated median and mean differences, correlations, and used the Passing and Bablok regression for comparisons. Concordance/discordance analyses were conducted. RESULTS: All equations provided generally accurate LDL-C estimations with slight differences among them. At TG<
1.69 mmol/L, no clinically significant divergences were observed. As TG values increased, LDL-C CONCLUSIONS: LDL-C