Strategic base modifications refine RNA function and reduce CRISPR-Cas9 off-targets.

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Tác giả: Siqi Huang, Xingyu Liu, Qianqian Qi, Wei Shen, Tian Tian, Xinyan Xu, Kaisong Zhang, Yunting Zhao, Xiang Zhou

Ngôn ngữ: eng

Ký hiệu phân loại: 972.8202 *Central America

Thông tin xuất bản: England : Nucleic acids research , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 187899

In contrast to traditional RNA regulatory approaches that modify the 2'-OH group, this study explores strategic base modifications using 5-carboxylcytosine (ca5C). We developed a technique where ca5C is transformed into dihydrouracil via treatment with borane-pyridine complex or 2-picoline borane complex, leading to base mutations that directly impact RNA functionality. This innovative strategy effectively manages CRISPR-Cas9 system activities, significantly minimizing off-target effects. Our approach not only demonstrates a significant advancement in RNA manipulation but also offers a new method for the precise control of gene editing technologies, showcasing its potential for broad application in chemical biology.
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