Chinese cabbage is an important vegetable in southern China. Excessive nitrogen fertilizer application can lead to the accumulation of nitrate in edible organs, which affects food value. Hence, the cultivation of varieties with high nitrogen utilization efficiency (NUE) and low nitrate accumulation is essential for molecular breeding. In flowering Chinese cabbage, Ammonium transporter 1
2 (AMT1
2,XM_009113156.2) significantly promotes plant growth, while reducing the nitrate content and ultimately improving the nutritional value of the crop
however, the exact underlying regulatory mechanisms remain unclear. Here, we aimed to investigate the response pattern of BcAMT1
2 to nitrogen (N) deficiency and mixed ammonium-nitrate nutrition and the potential roles played by its interacting proteins, Lateral organ boundaries domain 41 (LBD41,XM_009120072.3) and Membrane-anchored MYB (MAMYB,XM_009103351.3), in N metabolism. We found that transient silencing and overexpression of BcAMT1
2 regulated the absorption and accumulation of ammonium (NH