Engineering high-activity crosslinked enzyme aggregates via SpyCatcher/SpyTag-mediated self-assembly.

• Tác giả: Shibo Hao, Lili Jia, Xueliang Ma, Manzhu Wang, Yu Yang, Yubo Zhang, Bingxuan Zhao
• Ngôn ngữ: eng
• Ký hiệu phân loại: 133.594 Types or schools of astrology originating in or associated with a
• Thông tin xuất bản: Netherlands : International journal of biological macromolecules , 2025
• Bộ sưu tập: NCBI
• ID: 190217

Tóm tắt

Crosslinked Enzyme Aggregates (CLEAs) are favored for their operational stability and recyclability. However, the traditional CLEAs preparation may distort the enzyme's active site and reduce activity. Therefore, we developed a universally applicable crosslinked SpyCatcher scaffold system designed for the facile preparation of CLEAs. Four lysine residues were introduced to the N-terminus of SpyCatcher to enhance the crosslinking selectivity with glutaraldehyde. This scaffold subsequently enables the assembly of enzymes through the specific binding affinity between SpyTag and SpyCatcher. By precipitating SpyCatcher with (NH