An Efficient and Cost-Effective Novel Strategy for Identifying CRISPR-Cas-Mediated Mutants in Plant Offspring.

• Tác giả: Ying Fu, Li Huang, Meng Li, Xinyue Liang, Xueting Liu, Qian Shen, Sen Zhang, Wei Zhang
• Ngôn ngữ: eng
• Thông tin xuất bản: United States : The CRISPR journal , 2025
• Bộ sưu tập: NCBI
• ID: 190340

Tóm tắt

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 system has revolutionized targeted mutagenesis, but screening for mutations in large sample pools can be time-consuming and costly. We present an efficient and cost-effective polymerase chain reaction (PCR)-based strategy for identifying edited mutants in the T