OBJECTIVES: To clarify the association between telework with family members at home and work functioning impairment. METHODS: A cross-sectional survey was conducted in 27,036 participants. Having children or cohabitants in need of care at home during telework was considered an exposure factor. Multilevel logistic regression analysis was used to calculate odds ratios (ORs) for work functioning impairment, assessed by The Work Functioning Impairment Scale (WFun). RESULTS: Participants living with cohabitants in need of care at home had higher odds of work functioning impairment, whereas those living with preschool children or a spouse had lower odds. Participants living with children or cohabitants in need of care at home during telework had higher odds. CONCLUSIONS: We found an association between having children or cohabitants in need of care at home during telework and work functioning impairment.