Most cell types are mechanosensitive, their activities such as differentiation, proliferation and apoptosis, can be influenced by the mechanical environment through mechanical stimulation. In three dimensional (3D) mechanobiological in vitro studies, the porous structure of scaffold controls the local mechanical environment that applied to cells. Many previous studies have focused on the topological design of homogeneous scaffold struts. However, the impact of scaffold inhomogeneity on the mechanical environment, which is essential in mechanobiological application (e.g. for multi-cells co-culture), remains elusive. In this study, we use a computational fluid dynamics (CFD) approach together with data analysis to study the influence of a porosity gradient (10 %-30 % porosity difference) on the local and global mechanical environment (wall shear stress - WSS) within the commonly used structures of triple periodic minimal surfaces (TPMS). In addition, the anisotropy of internal WSS and scaffold permeability caused by the porosity gradient is investigated. It is found that the influence of anisotropy on the average WSS and permeability is up to 11 % and 31 %, respectively. These results, as theoretical references will be useful to tissue engineers and mechanobiologists for scaffold design and in vitro experiment planning such as integrated use of graded scaffold and bioreactors for specific cell types.