Gibberellin (GA) is one of the crucial plant hormones involved in fruit ripening regulation. GASA genes, which respond to GA and encode cysteine-rich peptides, are prevalent in plants. While the GASA gene family has been identified in various plants, its role in persimmon fruit ripening remains unclear. In this study, the GASA genes of persimmon were identified and analyzed. We found that gibberellin treatment suppresses the expression of the DkGASA4. Additionally, through dual-luciferase and yeast two-hybrid assays, we discovered that DkGASA4 interacts with DkNAC9 to synergistically activate the transcription of the cell wall degradation gene DkEGase1. This study highlights the significant regulatory function of DkGASA4 in persimmon fruit ripening and softening.