Lactic acid bacteria (LAB) are well-known for its expertise in synthesizing exopolysaccharides (EPS), which are linked to significant health benefits, such as its prebiotic effects and ability to modulate the immune system. However, the synthesis of EPS is hindered by low yields. The objective of this study was to investigate the impact of co-cultivation on EPS output by Weissella confusa XG-3 when paired with Candida shehatae. The structure and characteristics of the purified EPS were analyzed. Gel permeation chromatography (GPC) revealed a molecular weight of 3.45 × 10