The accurate detection of carbamate pesticides popularly employed in agricultural products is critical for reducing the threat of resultant residues to human health. In this work, a regenerable nanofilm used for SERS substrate was constructed by interfacially confined self-assembly incorporating CdS nanowires (CdSNWs) and Ag nanoparticles (AgNPs). The constructed AgNPs-CdSNWs/Nanofilm could significantly enhance the Raman signals of three carbamate pesticides (metolcarb, carbaryl and aldicarb-sulfone). A broad detection linear ranged 0.001-100 mg/L was achieved. The detection limits (LOD) of metolcarb, carbaryl and aldicarb-sulfone reached 0.00081 mg/L, 0.00081 mg/L and 0.00072 mg/L with coefficients of determination of (R