Imazaquin (IMQ) is an imidazolinone group herbicide widely used for weed control around the world. Due to excessive use during crop production, IMQ can accumulate in corn and soybeans, positing a potential threat to human health. In this study, a hapten that had high specificity and sensitivity was designed using computer-simulated technology. A monoclonal antibody (mAb) was synthesized with a half inhibitory concentration of 0.98 ng mL