L-Asparaginase CLEAs were prepared utilizing sodium tripolyphosphate (TPP) as a crosslinker (TA-CLEA). Under optimized conditions (pH 3, 0.3% TPP concentration, and a crosslinking time of 1 h), an 85% activity recovery was achieved. TA-CLEAs demonstrated superior pH stability (pH 3-8) compared to GA (glutaraldehyde)-CLEA but lost structural integrity at pH 9. TA-CLEAs were thermally more stable (concerning activity) and structurally less stable than GA-CLEA owing to the presence of weaker ionic bonds. TA-CLEAs reported an increase in apparent K