Long-term cadmium (Cd) exposure inhibits plant growth and development, reduces crop yield and quality, and threatens food security. Exploring the Cd tolerance mechanisms and safe production of crops in Cd-contaminated environment has become a worldwide concern. In this study, mung bean (Vigna radiata L.) cultivar Sulu (SL) and its three mutant lines (20#, 09#, and 06#) were used to compare the difference in Cd absorption, accumulation, and tolerance through pot and field experiments. 20#, 09#, and 06# are Cd-tolerant germplasms of mung bean but exist in different Cd tolerance mechanisms, 20# exhibited the lowest Cd absorption capacity, 09# possessed lower Cd translocation capacity, while 06# accumulated more Cd in protoplasts. Mung bean germplasms with higher Cd tolerance generally showed lower absorption capacity and intracellular accumulation of Cd. Besides, Cd accumulation in mung bean seeds is mainly depended on the absorption and translocation of Cd in roots and the Cd concentration in leaves, exogenous Mn supply inhibited the Cd