Saline-alkaline stress has caused severe ecological and environmental problems. Castor bean is a potential alkali-tolerant plant, however, its reactive oxygen species (ROS) regulatory mechanisms under alkaline stress remain unclear. This study investigated the physiological, transcriptomic, and metabolomic characteristics of two varieties (ZB8, alkaline-sensitive
JX22, alkaline-resistant) under alkaline stress. Results showed that under alkaline stress, JX22's root length was 1.66-fold greater than ZB8's, while its superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities were 1.25-, 1.41-, and 1.29-fold higher than ZB8's, respectively. The levels of superoxide anion (O