Soil dust in infrastructure construction sites seriously affects the working environment. In the study, urease is a biological enzyme extracted from jack bean, which is used as the core material to prepare dust suppressant. The best urease activity was observed at bean flour mass to Citric Acid-Sodium Citrate (CASC) volume ratio of 5:14. Urease activity increased with temperature rise and showed a trend of first increasing then decreasing with pH increase, with highest activity at pH 8. The precipitation rate of CaCO