OBJECTIVE: To introduce a novel technique, dynamic MATERIALS AND METHODS: DyMAG3 SPECT/CT was applied to nine index cases diagnosed with conditions such as ureteropelvic junction obstruction, ureterovesical junction obstruction, or hydronephrosis resembling ureteropelvic junction obstruction, with or without renal parenchymal thinning. Imaging was conducted just before planned pyeloplasty or ureteroneocystostomy. After a 0.1 mCi/kg RESULTS: Preliminary findings from the first nine cases revealed that DyMAG3 SPECT/CT could provide both anatomical and functional data obtained equivalent to conventional techniques in a single imaging session. Moreover, DyMAG3 SPECT/CT improved the diagnostic abilities of CONCLUSION: This preliminary study suggests that DyMAG3 SPECT/CT could emerge as a state-of-the-art tool for the preoperative evaluation of obstructive uropathies.