Stingless bee honey (SBH) has garnered attention as a natural sweetener and functional food, recognized for its exceptional biological activity and distinctive flavour. Compared to honey bee honey, SBH has lower annual production but commands a higher market value, making it more susceptible to adulteration. During an analysis of trehalulose in SBH, we unexpectedly identified the naturally occurring sugar alcohol, mannitol. The presence of mannitol in SBH was confirmed using UHPLC/Q-TOF-MS,