Education plays a critical role on promoting preventive behaviours against the spread of pandemics. In Japan, hand-washing education in primary schools was positively correlated with preventive behaviours against COVID-19 transmission for adults in 2020 during the early stages of COVID-19 [1]. The following year, the Tokyo Olympics were held in Japan, and a state of emergency was declared several times. Public perceptions of and risks associated with the pandemic changed drastically with the emergence of COVID-19 vaccines. We re-examine whether effect of hand-washing education on preventive behaviours persisted by covering a longer period of the COVID-19 pandemic than previous studies. 26 surveys were conducted nearly once a month for 30 months from March 2020 (the early stage of COVID-19) to September 2022 in Japan. By corresponding with the same individuals across surveys, we comprehensively gathered data on preventive behaviours during this period. In addition, we asked about hand-washing education they had received in their primary school. We used the data to investigate how and the degree to which school education is associated with pandemic mitigating preventive behaviours. We found that hand-washing education in primary school is positively associated with behaviours such as hand washing and mask wearing as a COVID-19 preventive measure, but not related to staying at home. We observed a statistically significant difference in hand washing between adults who received childhood hand-washing education and those who did not. This difference persisted throughout the study period. In comparison, the difference in mask wearing between the two groups was smaller, but still statistically significant. Furthermore, there was no difference in staying at home between them.