BACKGROUND: Gestational weight gain (GWG) plays a critical role in determining birth outcomes, especially in twin pregnancies. However, the association between GWG and adverse birth outcomes in twin pregnancies remains inconclusive. This study aims to define GWG according to different classification criteria and explore their associations with adverse birth outcomes in twin pregnancies. METHODS: This was a prospective cohort study that included 1,029 twin pregnant women recruited from Qingdao Women and Children's Hospital between September 2018 and December 2020. Participants were categorized into insufficient, adequate, and excessive GWG groups using both the interquartile range (P25-P75) method and the Institute of Medicine (IOM) criteria. Logistic regression models were employed to assess the associations between GWG and adverse birth outcomes, including preterm birth (PTB), low birth weight (LBW), and small for gestational age (SGA). RESULTS: According to the interquartile range method, women in the insufficient GWG group had a significantly increased risk of PTB (OR: 2.13, 95% CI: 1.55-2.94), LBW (OR: 2.01, 95% CI: 1.33-3.05), and SGA (OR: 1.62, 95% CI: 1.03-2.54) compared to adequate GWG group. In contrast, the excessive GWG group was associated with a reduced risk of LBW (OR: 0.64, 95% CI: 0.45-0.92) and SGA (OR: 0.51, 95% CI: 0.28-0.91) after adjusting for potential confounders. Similar trends were observed using the IOM criteria, with a significantly increased risk of PTB and LBW in twin pregnant women with insufficient GWG and a reduced risk of SGA with excessive GWG. CONCLUSIONS: Achieving an appropriate level of weight gain during pregnancy is essential to reduce the risk of adverse birth outcomes in women with twin pregnancies.