BACKGROUND: Chagas disease (CD), caused by METHODS: Cruzain and AsCystatin were obtained recombinantly through expression in RESULTS: AsCystatin, a potential inhibitor of cysteine proteases, was identified from previously published scientific literature. CONCLUSION: It was possible to identify AsCystatin and four peptides derived from this protein with inhibitory activity on cruzain, highlighting the trypanocidal effect of these peptides observed in