Cell Sizes Matter for Industrial Bioproduction, a Case of Polyhydroxybutyrate.

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Tác giả: Guo-Qiang Chen, Yi-Ling Chen, Wei-Ke Guo, Xu Liu, Jia-Le Wang, Fu-Qing Wu, Qiong Wu, Xu Yan, Ji-Shuai Yang, Li-Zhan Zhang, Shuang Zheng

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Germany : Advanced science (Weinheim, Baden-Wurttemberg, Germany) , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 198991

Most bacterial cells are 1-2 microns in size, limiting intracellular products like polyhydroxyalkanoates (PHA) accumulation. Cell size is regulated by key genes such as mreB and minCD, which encode cellular skeleton protein and control cell fission ring location, respectively. Their expression changes significantly affect microbial growth. This study successfully redesigns the ClpXP protein degradation system by deleting the sspB gene and using mutated SsrA tags with different degradation rates to control MreB degradation. Dynamic degradation of MreB allows non-model bacterium Halomonas bluephagenesis to grow normally and increase cell size simultaneously. Combined with overexpression of minCD, H. bluephagenesis with progressive MreB degradation increases the cell size further, albeit with a reduced growth rate. H. bluephagenesis CYL0307, with the PHB granule-associated protein PhaP1 deleted and phaAB
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