Heterologous expression is crucial to produce various recombinants proteins, yet consistently achieving high yields poses a significant challenge. The main objective of our research was to engineer the cell membrane components of Bacillus licheniformis for improving heterologous proteins production. This engineering strategy was achieved by overexpressing genes bkdR, plsY, plsC, and deleting pssA and clsA, which significantly increased the production of nattokinase, α-amylase and keratinase. Furthermore, a combined engineered strain was constructed by integrating all these approaches into a single strain (DW2-RYCAS) which led to an increase in the negative charge and permeability of the cell membrane by 41.11 % and 57.62 %, respectively, and reduced cell membrane integrity by 81.45 % compared to the control strain DW2. Ultimately, the production of nattokinase, α-amylase, and keratinase in DW2-RYCAS were 406.02 ± 8.17 FU/mL, 526.80 ± 14.77 U/mL, and 18.27 ± 0.70 KU/mL, respectively, which increased by 493.59 %, 273.40 %, and 213.91 % compared to the control strain DW2. These results represent the highest production of nattokinase, α-amylase, and keratinase in shake flasks reported to date. Our research illustrated the promising application of cell membrane engineering in B. licheniformis, creating an excellent platform for the biosynthesis of heterologous proteins.