BACKGROUND: High-intensity interval training (HIIT) has been recognized as an effective form of short-duration exercise. The purpose of this study was to assess whether HIIT could reduce renal injury induced by myocardial ischemia-reperfusion (MIR) in rats. METHODS: Male Sprague-Dawley rats were randomly assigned to the Sham (SHAM), coronary artery occlusion (CAO), HIIT, and ischemic precondition (IPC) groups. Rats underwent 40 minutes of left anterior descending CAO under anesthesia, followed by 3 hours of reperfusion, to induce MIR. Postsurgery, rats were sacrificed, and their blood, heart, and kidney tissues were examined. The HIIT group underwent 4 weeks of HIIT training before surgery. RESULTS: HIIT intervention significantly reduced renal injury after MIR and the concentrations of blood urea nitrogen (BUN) and creatinine (CRE) in the serum. Moreover, proinflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6, were significantly decreased, while the anti-inflammatory cytokine IL-10 was significantly increased in the serum. Additionally, HIIT intervention suppressed the expression of FoxO1, Bax/Bcl-2 ratio, TNF-α, and cleaved-caspase-3/caspase-3 ratio in kidney tissues, ultimately reducing renal cell apoptosis. CONCLUSION: This study is the first to demonstrate that HIIT has effects similar to IPC, significantly reducing renal injury after MIR. HIIT regulates the production of proinflammatory and anti-inflammatory cytokines and inhibits renal cell apoptosis, thereby reducing the occurrence of cardiorenal syndrome.