OBJECTIVES: This study evaluated the impact of increased working from home on musculoskeletal pain before and after the SARS-CoV-2 pandemic. METHODS: In an online survey (September 2023 to April 2024), pain was rated on a numeric rating scale. The impact of working from home and covariates on new pain onset and exacerbation was modeled using logistic regression analyses. RESULTS: Of 1,064 participating computer workers, 968 were working from home. Working from home compared to office-only work, showed a trend of increasing pain. Longer remote working hours and poorer workstation setups also increased the risks for pain (e.g., new neck or upper back pain OR 2.02, 95% CI 1.08-3.76). CONCLUSIONS: Preventing musculoskeletal pain should involve improving remote workstation ergonomics, promoting healthy lifestyles, participation in regular occupational health screenings, and supporting employees with anxiety or depression symptoms.