The Pacific Symposium on Biocomputing (PSB) 2014 is an international, multidisciplinary conference for the presentation and discussion of current research in the theory and application of computational methods in problems of biological significance. Presentations are rigorously peer reviewed and are published in an archival proceedings volume. PSB 2014 will be held from January 3 - 7, 2014 in Kohala Coast, Hawaii. Tutorials and workshops will be offered prior to the start of the conference. PSB 2014 will bring together top researchers from the US, the Asian Pacific nations, and around the world to exchange research results and address open issues in all aspects of computational biology. It is a forum for the presentation of work in databases, algorithms, interfaces, visualization, modeling, and other computational methods, as applied to biological problems, with emphasis on applications in data-rich areas of molecular biology. The PSB has been designed to be responsive to the need for critical mass in sub-disciplines within biocomputing. For that reason, it is the only meeting whose sessions are defined dynamically each year in response to specific proposals. PSB sessions are organized by leaders of research in biocomputing's "hot topics." In this way, the meeting provides an early forum for serious examination of emerging methods and approaches in this rapidly changing field. Contents: Cancer Panomics: Computational Methods and Infrastructure for Integrative Analysis of Cancer High-Throughput "Omics" Data Computational Approaches to Drug Repurposing and Pharmacology Detecting and Characterizing Pleiotropy: New Methods for Uncovering the Connection Between the Complexity of Genomic Architecture and Multiple Phenotypes Personalized Medicine: From Genotypes and Molecular Phenotypes Towards Therapy Text and Data Mining for Biomedical Discovery Workshops Readership: Academia and industry in the fields of biocomputing, bioinformatics and computational biology.