Determination of the minimum postmortem interval remains an important factor in the investigation of deaths, especially homicides. It is important for near-accurate reconstruction of the fatal event, especially after putrefaction has commenced. It helps to weigh the alibi of suspects, and to include or exclude these individuals. The science of forensic entomology, acarology, palynology and taphonomy have been applied with varying degree of accuracy depending on a plethora of biotic and abiotic factors. Each of these specialties is most useful at specific times during decomposition and depending on local environmental factors. As decomposition progresses, the estimation of the PMI becomes more difficult. Here, we review how different forensic techniques are most appropriate for estimating the postmortem interval at different times since death. Ultimately, the determination of the interval is a difficult task requiring a multidisciplinary approach comprising many forensic specialists.