DNA Polymerases in Biotechnology

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Tác giả: Andrew F. Gardner, Zvi Kelman

Ngôn ngữ: eng

ISBN-13: 978-2889194551

Ký hiệu phân loại:

Thông tin xuất bản: Frontiers Media SA, 2015

Mô tả vật lý: 1 electronic resource (146 p.)

Bộ sưu tập: Tài liệu truy cập mở

ID: 202237

DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology. For example, DNA polymerases have been engineered for increased speed and fidelity in PCR while lowering amplification sequence bias. Inhibitor resistant DNA polymerase variants enable PCR directly from tissue (i.e. blood). Design of DNA polymerases that efficiently incorporate modified nucleotide have been critical for development of next generation DNA sequencing, synthetic biology and other labeling and detection technologies. The Frontiers in Microbiology Research Topic on DNA polymerases in Biotechnology aims to capture current research on DNA polymerases and their use in emerging technologies.
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