An origin discrimination model for rice from five production regions in Heilongjiang Province was constructed based on the combination of confocal microscopy Raman spectroscopy and chemometrics. A total of 150 field rice samples were collected from the Fangzheng, Chahayang, Jiansanjiang, Xiangshui, and Wuchang production areas. The optimal sample processing conditions, instrument parameter settings, and spectrum acquisition techniques were identified by investigating the influencing factor. The Raman spectra of milled rice within the range of 100-3200 cm