Six potato cultivars developed in Lithuania, 'VB Venta', 'Goda', 'VB Liepa', 'VB Meda', 'VB Rasa' and 'VB Aista', were selected based on the results of trials in the Ukrainian climatic zone from 2020--2021. Three of the cultivars, 'VB Goda', 'VB Liepa', and 'VB Meda', presented elevated levels of resistance to Synchytrium endobioticum (Schilbersky) Percival and Phytophthora infestans (Mont) de Bary. These cultivars are recommended for use as parental lines for crossing and as sources of disease-resistant potatoes. Simple sequence repeat (SSR) markers are frequently employed in breeding and genetic studies because of their prevalence, repetition, and extensive genome coverage. These results indicate that Lithuanian potato cultivars exhibit significant genetic differences, which also translate into distinct agromorphological characteristics.