Bioluminescence : Recent Advances in Oceanic Measurements and Laboratory Applications

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Tác giả: David Lapota

Ngôn ngữ: eng

ISBN-13: 978-9535152026

Ký hiệu phân loại: 664.0282028 Food technology

Thông tin xuất bản: Croatia : IntechOpen, 2012

Mô tả vật lý: 1 electronic resource (202 p.)

Bộ sưu tập: Tài liệu truy cập mở

ID: 207220

We now find ourselves utilizing luciferase - luciferin proteins, ATP, genes and the whole complex of these interactions to observe and follow the progress or inhibition of tumors in animal models by measuring bioluminescence intensity, spatially and temporally using highly sophisticated camera systems. This book describes applications in preclinical oncology research by bioluminescence imaging (BLI) with a variety of applications.
- Chapter s describe current methodologies for rapid detection of contaminants using the Milliflex system, and the use of bioluminescence resonance energy transfer (BRET) technology for monitoring physical interactions between proteins in living cells. Others are using bioluminescent proteins for high sensitive optical reporters imaging in living animals, developing pH-tolerant luciferase for brighter in vivo imaging, and oscillation characteristics in bacterial bioluminescence. The book also contains descriptions of the long-term seasonal characteristics of oceanic bioluminescence and the responsible planktonic species producing bioluminescence. Such studies are few and rare.
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