This study was designed to investigate the promotive effect of transglutaminase (TG) on the gelling properties and sensorial attributes of lysine (Lys)-rich salt-reduced frankfurters. The results revealed that the addition of 0.3% TG yielded acceptable cooking loss and emulsion stability in Lys-rich salt-reduced frankfurters, significantly improved their textural parameters, and visibly optimised their microstructure. Moreover, combination treatment with TG and Lys also promoted the gelling properties of the thermally induced gels, conferring greater viscoelasticity and stability. Meanwhile, TG and Lys positively transformed their protein conformations by promoting the generation of isopeptide bonds and enhancing the predominant molecular forces (hydrogen bonds and hydrophobic interactions). Additionally, combination treatment with 0.3% TG and Lys increased the overall sensory score of the salt-reduced frankfurters from 45.4 to 82.6. In summary, the combined application of TG and Lys potentially serves as an efficient salt-replacement strategy in emulsified meat products, providing superior product quality and health benefits.