As an omnipresent occurrence in the natural world, chirality plays a crucial role in numerous biological and physiological processes. Therefore, incorporation of chirality into biointerface materials has been emerging as a research hotspot in the development of regenerative biomaterials. Nevertheless, how chiral biointerface materials interact with biological organisms remains poorly understood. In the current study, a bioinspired chiral self-assembled nanohydroxyapatite material is developed for bone regeneration using a distraction osteogenesis model. We found that the left-handed chirality, rather than the right-handed chirality, possessed the greatest heightened ability for bone regeneration. Molecular mechanism research reveals that the left-handed chirality can activate mechanosensitive pathways to promote Ca