Effects of basic fibroblast growth factor on cartilage to bone: Time-course histological analysis of

• Tác giả: Rin Amagase, Michiyo Nasu, Shinichiro Takayama, Akihiro Umezawa
• Ngôn ngữ: eng
• Ký hiệu phân loại: 519.5354 Statistical mathematics
• Thông tin xuất bản: Netherlands : Regenerative therapy , 2025
• Bộ sưu tập: NCBI
• ID: 209801

Tóm tắt

INTRODUCTION: Cartilage tissue is important in the human body as a ubiquitous connective tissue. However, information on the limits of chondrocyte growth and the effects of basic FGF (bFGF) METHODS: Chondrocytes were isolated from the epiphyseal cartilage derived from the digits of polydactyly patients and were cultivated in a medium supplemented with bFGF (bFGF-treated cells) and without bFGF (bFGF-untreated cells). These cultivated cells were subcutaneously implanted into the backs of NOG mice. RESULTS: bFGF-treated cells exhibited a higher growth rate than bFGF-untreated cells. Cartilage was formed after two weeks of implantation. The cartilages generated by bFGF-treated cells (denoted as "BT-cartilage" hereafter) were larger in size and heavier in weight than those cartilages generated by bFGF-untreated cells (denoted as "BUT-cartilage" hereafter). BT-cartilage grew exponentially after 10 weeks. CONCLUSIONS: From these results, bFGF-treated cells exhibited increased ability for both proliferation and differentiation compared to bFGF-untreated cells. The results of this study may lead to the generation of new alternative therapies for bone and cartilage through the use of epiphyseal chondrocytes.