Monitoring pesticide residues in soil is crucial for ensuring food safety and environmental sustainability. Spinosad, widely used in sustainable agriculture due to its selective toxicity and reduced environmental impact, poses detection challenges with traditional chromatographic methods, which require extensive sample preparation and are destructive. This study evaluates quantitative nuclear magnetic resonance (qNMR) as a non-destructive, efficient method for spinosad quantification in soil samples, emphasizing its potential for routine environmental monitoring. The qNMR method was validated with an 88% recovery rate for spinosad in agricultural soils, a limit of detection (LOD) of 0.0414 mg mL