The assessment of blueberry fruit quality is traditionally conducted through laboratory equipment. Despite its high accuracy, this method remains destructive, labor-intensive, time-consuming, and costly. Consequently, there is a pressing need for innovative solutions such as image-based and artificial intelligence (AI)-driven analysis. To address these limitations, this study aimed to analyze whether an approach based on mobile image-based analysis combined with machine learning (ML) algorithms could develop a non-destructive framework for evaluating blueberry fruit quality, specifically focusing on total soluble solids (TSS) and firmness. Firstly, we collected numerous blueberry samples during the maturity stage to construct a comprehensive dataset. These samples were meticulously analyzed in a laboratory for diameter, TSS, firmness, and color. Simultaneously, RGB images were captured using a mobile device. These images were processed to extract spectral bands (red, green, and blue). Eight ML algorithms were employed to develop predictive models capable of predicting the qualitative parameters of the blueberries. Initially, correlation analysis demonstrated that RGB images suggestively contribute to fruit quality assessment (r <
0.41). However, the integration of ML algorithms significantly enhanced the predictive accuracy of these models (R