Auricular defects are highly prevalent and have a significant impact on the physical and mental well-being of patients. However, due to the intricate anatomy of the auricle, achieving personalized and precise reconstruction poses a major challenge. Currently, tissue engineering auricle scaffolds based on rigid materials are an effective therapeutic approach for auricle reconstruction. Nevertheless, these auricular scaffolds often fail to meet biomechanical requirements and lack biological activity, resulting in suboptimal treatment outcomes. In this study, polyvinyl alcohol and gelatin were used as printing inks, and nano-silica was employed as a filler to optimize the printability of the inks. Through layer-by-layer 3D printing, auricle scaffolds were fabricated that closely mimic human auricular biomechanical properties and possess a multi-scale pore structure. Subsequent in vitro experiments confirmed the biocompatibility of the scaffolds. Furthermore, a rabbit auricular cartilage defect model was established to evaluate the therapeutic efficacy of this bionic scaffold featuring a multi-scale pore structure for auricle defects. The findings demonstrated that the developed auricle scaffold not only exhibited excellent biomechanical strength and favorable biocompatibility but also provided an advantageous environment for chondrocyte growth due to its multi-scale pore structure, thereby significantly promoting chondrocyte proliferation. Overall, the 3D printed tissue engineering bionic scaffold with a multi-scale pore structure developed in this study is anticipated to significantly enhance the therapeutic efficacy for auricle defects and offer a novel therapeutic strategy for such defects.