Long non-coding RNA AK007111 mediates mast cells apoptosis via targeting of protein MOAP1.

• Tác giả: Feng Liu, Yingying Luo, Xiao Ma, Heng Tang, Changdi Xu, Jiejing Xu
• Ngôn ngữ: eng
• Ký hiệu phân loại: 612.8262 Nervous system Sensory functions
• Thông tin xuất bản: England : The Journal of asthma : official journal of the Association for the Care of Asthma , 2025
• Bộ sưu tập: NCBI
• ID: 214015

Tóm tắt

BACKGROUND: Long non-coding RNAs (lncRNAs) are emerging regulators of pathophysiological processes in a variety of diseases including asthma. In this study, we reported the identification of lncRNA-AK007111 as an essential modulator of mast cell apoptosis and investigated its potential mechanism. METHODS: RNA-seq profiling and transcriptome sequencing technology were adopted to screen for differentially expressed genes. Transfection was done by small interfering RNAs (siRNAs) to down-regulate lncRNA-AK007111 and Modulator of apoptosis 1 (MOAP1). Starvation was used to induce apoptosis. The apoptotic rate was measured by flow cytometry. Western Blot was conducted to detect the expression of apoptosis-related proteins. RESULTS: LncRNA-AK007111 was highly expressed in IgE/Ag-mediated activation of mast cells. Down-regulation of LncRNA-AK007111 promoted apoptosis of mast cells. Down-regulation of MOAP1 attenuated apoptosis in mast cells induced by the down-regulation of lncRNA-AK007111. CONCLUSION: LncRNA-AK007111 may be a potential regulator of mast cell apoptosis by interaction with MOAP1.