Chondritic meteorites can be appropriate substrates for the colonization of terrestrial microorganisms. However, determining whether organic compounds are intrinsic to the meteorite or come from external (terrestrial) contamination is still controversial. This research explores the molecular distribution and carbon isotopic composition of three lipid families (hydrocarbons, alkanoic acids, and alcohols) as well as DNA extracted from the interior of a CO carbonaceous chondrite named El Médano 464 (EM 464), discovered in the Atacama Desert in 2019. Three soil samples from the discovery area of EM 464 were collected and used as a background control for the composition and distribution of organic compounds. Our results revealed a higher abundance of the three lipid families in EM 464 compared with the surrounding soil samples. The organic compounds in EM 464 showed a mean δ