BACKGROUND: Djon Djon is a particularly special black mushroom indigenous to Haiti that has a long history in both their cuisine and in traditional medicine. Centuries of folkloric utilization of theses "medicinal" botanicals tend to indicate the presence of a potentially efficacious western medicine entity. OBJECTIVES: With the advantages afforded by both traditional medicines and fermentation, we endeavored to investigate if fermentation of Djon Djon mushrooms can provide skin care benefits. METHODS: In this study, active Djon Djon fermentation broth (DDF) was obtained using Saccharomyces, and anti-inflammatory efficacy was assessed in cultured systems using human keratinocytes and fibroblasts, exposed to either UVB or H RESULTS: Characterization of the DDF displayed a high number of polysaccharides and peptides present following fermentation, that function to scavenge intracellular ROS, decrease MDA content, while increasing the levels of CAT, COL-I, and HA in HSF induced by H CONCLUSION: DDF can alleviate oxidative stress damage caused by hydrogen peroxide and photodamage caused by UVB, and the mechanism by which DDF protects skin cells is revealed, displaying the potential benefits of fermented DjonDjon in skincare.