UNLABELLED: Viable pollen is crucial for fertilization, but pollen is generally highly susceptible to heat stress. A quick, reliable method for testing the heat-stress tolerance of pollen is needed to improve the heat-stress tolerance in plants, but current methods require considerable space and labor. In addition, many such methods only test tolerance to a single constant temperature, making it time-consuming to screen heat tolerance over a wide temperature range and to examine the dynamics of pollen viability at different temperatures. To address this issue, we aimed to: (1) develop an easy, reliable method for measuring pollen viability at different temperatures
and (2) identify the best temperature range for screening pollen with high heat-stress tolerance. We harvested mature pollen from wheat ( SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s42994-024-00183-3.